Genetics and Bioengineering (English) | |||||
Bachelor | TR-NQF-HE: Level 6 | QF-EHEA: First Cycle | EQF-LLL: Level 6 |
Course Code: | GBE202 | ||||||||
Course Name: | Bioengineering Laboratory | ||||||||
Course Semester: | Spring | ||||||||
Course Credits: |
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Language of instruction: | EN | ||||||||
Course Requisites: | |||||||||
Does the Course Require Work Experience?: | No | ||||||||
Type of course: | Compulsory | ||||||||
Course Level: |
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Mode of Delivery: | Face to face | ||||||||
Course Coordinator : | Dr.Öğr.Üyesi FATMA TUBA AKDENİZ | ||||||||
Course Lecturer(s): |
Dr.Öğr.Üyesi FATMA TUBA AKDENİZ |
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Course Assistants: |
Course Objectives: | Teaching how a bioengineer works in the laboratory environment depending on general safety rules To teach the basic usage of molecular biology and bioengineering devices |
Course Content: | This course is designated to provide the introduction knowledge to laboratory techniques, scientific calculation methods, laboratory safety rules and basic tools of molecular biology and genetics. |
The students who have succeeded in this course;
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Week | Subject | Related Preparation |
1) | • Syllabus • Introduction of bioengineering laboratory • Discussing laboratory safety rules • Describing using basic laboratory instruments • Describing how to take notes in laboratories • Describing scientific reports and preparing scientific reports | |
2) | • Describing measurement uncertainty • Describing measurement with balances and microbalances • Calculations of measurement uncertainty of balances | |
3) | • Defining using microscopy • Defining how to use a microscope with pre-stained slides • Preparing epithelial sample from mouth and staining it with thryphan blue for microscope examination | |
4) | • Describe The Growth Mediums and The Buffers • Describe the mediums and the buffers • Concentration calculations • Preparing TBE buffer • Preparing LB and LB-Agar | |
5) | • Describe The Serial Dilution • Describe The Bacterial Cell Culture Methods • Define Gram Staining Methods • Calculations of different dilutions of bacterial culture • Describing different bacterial cell culture methods • Colony counting with pre-cultured bacterial cells • Gram staining with pre-cultured bacterial cells • Imaging of gram staining | |
6) | • Describe The Bacterial Growth • Define the Effects on Bacterial Growth as UV and High Temperature • Treating bacterial cells with UV and high temperature; keeping a group as control • Repeating UV-Spectrophotometer Method • Drawing bacterial growth curve for preset 3 groups of bacterial cell cultures | |
7) | Midterm week | |
8) | • Describe DNA Isolation From Bacterial Cells • Describe DNA Isolation From Blood • Describing basic steps of DNA isolation from bacterial cell culture • Describing basic steps of DNA isolation from blood | |
9) | • Describing polymerase chain reaction (PCR) technique • Defining each PCR component function in PCR amplification • Describing how to prepare PCR mix • Primer dilution • Discussion of importance of specific primer selection in PCR amplification • Adjustment of PCR temperature | |
10) | • Describe agarose gel electrophoresis technique • Preparations of PCR products for agarose gel electrophoresis • Preparations of agarose gel • Explain the role of loading dye and DNA marker • Loading and running of PCR products in agarose gel electrophoresis • Evaluating the results of agarose gel electrophoresis in UV light. • Evaluating DNA size and weight from agarose gel electrophoresis results | |
11) | • Describe plasmid isolation from microorganism • Explain the plasmid structure • Plasmid isolation components • Plasmid purification | |
12) | • Describe restriction, digestion and ligation process • Describe restriction enzymes • Describing digestion process • Defining ligases and ligation process • Controlling ligation products with agarose gel electrophoresis | |
13) | • Describing real-time quantitative PCR technique (qRT-PCR) • Describe imaging techniques in qRT-PCR-probes and dyes • Defining each qPCR component function in qPCR amplification • Describing how to prepare qPCR mix • Plotting a standard curve and normalization of the results • Interpretations of the qPCR results | |
14) | • Describe protein polyacrylamide gel electrophoresis • Denaturation of the protein • Preparation of polyacrylamide gel • Loading and running the protein in electrophoresis • Evaluating the weight of the protein with imaging the gel | |
15) | Final Exam |
Course Notes / Textbooks: | Lecture Notes |
References: | Ders Notlari |
Learning Outcomes | 1 |
2 |
3 |
4 |
5 |
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Program Outcomes | ||||||||||
1) Sufficient knowledge in mathematics, science and engineering related to their branches; and the ability to apply theoretical and practical knowledge in these areas to model and solve engineering problems. | ||||||||||
2) The ability to identify, formulate, and solve complex engineering problems; selecting and applying appropriate analysis and modeling methods for this purpose. | ||||||||||
3) The ability to design a complex system, process, device or product under realistic constraints and conditions to meet specific requirements; the ability to apply modern design methods for this purpose. (Realistic constraints and conditions include such issues as economy, environmental issues, sustainability, manufacturability, ethics, health, safety, social and political issues, according to the nature of design.) | ||||||||||
4) Ability to develop, select and use modern techniques and tools necessary for engineering applications; ability to use information technologies effectively. | ||||||||||
5) Ability to design experiments, conduct experiments, collect data, analyze and interpret results to examine engineering problems or discipline-specific research topics. | ||||||||||
6) The ability to work effectively in disciplinary and multidisciplinary teams; individual work skill. | ||||||||||
7) Effective communication skills in Turkish oral and written communication; at least one foreign language knowledge; ability to write effective reports and understand written reports, to prepare design and production reports, to make effective presentations, to give and receive clear and understandable instructions. | ||||||||||
8) Awareness of the need for lifelong learning; access to knowledge, ability to follow developments in science and technology, and constant self-renewal. | ||||||||||
9) Conform to ethical principles, and standards of professional and ethical responsibility; be informed about the standards used in engineering applications. | ||||||||||
10) Awareness of applications in business, such as project management, risk management and change management; awareness of entrepreneurship, and innovation; information about sustainable development. | ||||||||||
11) Information about the universal and social health, environmental and safety effects of engineering applications and the ways in which contemporary problems are reflected in the engineering field; awareness of the legal consequences of engineering solutions. |
No Effect | 1 Lowest | 2 Low | 3 Average | 4 High | 5 Highest |
Program Outcomes | Level of Contribution | |
1) | Sufficient knowledge in mathematics, science and engineering related to their branches; and the ability to apply theoretical and practical knowledge in these areas to model and solve engineering problems. | 1 |
2) | The ability to identify, formulate, and solve complex engineering problems; selecting and applying appropriate analysis and modeling methods for this purpose. | 1 |
3) | The ability to design a complex system, process, device or product under realistic constraints and conditions to meet specific requirements; the ability to apply modern design methods for this purpose. (Realistic constraints and conditions include such issues as economy, environmental issues, sustainability, manufacturability, ethics, health, safety, social and political issues, according to the nature of design.) | 1 |
4) | Ability to develop, select and use modern techniques and tools necessary for engineering applications; ability to use information technologies effectively. | 1 |
5) | Ability to design experiments, conduct experiments, collect data, analyze and interpret results to examine engineering problems or discipline-specific research topics. | 1 |
6) | The ability to work effectively in disciplinary and multidisciplinary teams; individual work skill. | 1 |
7) | Effective communication skills in Turkish oral and written communication; at least one foreign language knowledge; ability to write effective reports and understand written reports, to prepare design and production reports, to make effective presentations, to give and receive clear and understandable instructions. | 1 |
8) | Awareness of the need for lifelong learning; access to knowledge, ability to follow developments in science and technology, and constant self-renewal. | |
9) | Conform to ethical principles, and standards of professional and ethical responsibility; be informed about the standards used in engineering applications. | 1 |
10) | Awareness of applications in business, such as project management, risk management and change management; awareness of entrepreneurship, and innovation; information about sustainable development. | |
11) | Information about the universal and social health, environmental and safety effects of engineering applications and the ways in which contemporary problems are reflected in the engineering field; awareness of the legal consequences of engineering solutions. |
Expression | |
Brainstorming/ Six tihnking hats | |
Individual study and homework | |
Lesson | |
Lab | |
Homework | |
Report Writing |
Written Exam (Open-ended questions, multiple choice, true-false, matching, fill in the blanks, sequencing) | |
Homework | |
Application | |
Reporting |
Semester Requirements | Number of Activities | Level of Contribution |
Field Work | 5 | % 15 |
Midterms | 1 | % 30 |
Final | 1 | % 40 |
Paper Submission | 5 | % 15 |
total | % 100 | |
PERCENTAGE OF SEMESTER WORK | % 60 | |
PERCENTAGE OF FINAL WORK | % 40 | |
total | % 100 |
Activities | Number of Activities | Duration (Hours) | Workload |
Course Hours | 13 | 2 | 26 |
Laboratory | 13 | 2 | 26 |
Homework Assignments | 5 | 1 | 5 |
Quizzes | 5 | 1 | 5 |
Midterms | 1 | 2 | 2 |
Paper Submission | 5 | 1 | 5 |
Final | 1 | 2 | 2 |
Total Workload | 71 |