Medical Laboratory Technologies
Associate	TR-NQF-HE: Level 5	QF-EHEA: Short Cycle	EQF-LLL: Level 5

General course introduction information

Course Code:

TLT208

Course Name:

Molecular Biological Methods

Course Semester:

Fall

Course Credits:

Theoretical	Practical	Credit	ECTS
3	2	4	5

Language of instruction:

Course Requisites:

Does the Course Require Work Experience?:

Type of course:

Compulsory

Course Level:

Associate

TR-NQF-HE:5. Master`s Degree

QF-EHEA:Short Cycle

EQF-LLL:5. Master`s Degree

Mode of Delivery:

Face to face

Course Coordinator :

Dr.Öğr.Üyesi DİDEM ÖZKAN

Course Lecturer(s):

Dr.Öğr.Üyesi DİDEM ÖZKAN
Dr.Öğr.Üyesi FAZİLET ÖZDENOĞLU

Course Assistants:

Course Objective and Content

Course Objectives:	The aim of the course is to teach students; the structure of DNA, the structure of RNA, DNA and RNA Isolation Methods, Polymerase Chain Reaction and Strips, Electrophoresis and Thrips, Blotloma Techniques, Introduction to Cell Culture, Proteomics, Transcryptomics.
Course Content:	Structure of DNA The structure of RNA, DNA and RNA Isolation Methods, Polymerase Chain Reactions Electrophoresis Blotloma Techniques, Introduction to Cell Culture, Cell Passage and Maintenance Proteomics Technology Genomics and Transcriptomics Technologies

Learning Outcomes

The students who have succeeded in this course;

Learning Outcomes

1 - Knowledge
Theoretical - Conceptual
1) Possess theoretical and practical knowledge supported by textbooks with updated information, practice equipments and other resource on basic level based on qualifications gained at secondary education level.
2 - Skills
Cognitive - Practical
1) Gain the skills to use basic level theoretical and practical knowledge acquired within the field in the same field of a higher education level or in a field of same level.
2) Interpret and evaluate data, define problems, do analysis, produce solutions based on proof with using basic level knowledge and practices gained within the field.
3 - Competences
Communication and Social Competence
Learning Competence
Field Specific Competence
Competence to Work Independently and Take Responsibility

Lesson Plan

Week	Subject	Related Preparation
1)	General information about the course content
2)	Structure of DNA Structure of RNA General information about nucleic acids
3)	Isolatıon Methods DNA Extraction of Blood DNA Extraction of Cell Extraction of Tissue
4)	Polymerase chain reaction Polymerase chain reaction conditions Polymerase chain reaction devices
5)	Electrophoresis Application of Electrophoresisc
6)	Applications of Horizontal Electrophoresis Applications of Vertical Electrophoresis
7)	Blot Technics
8)	Mid Term Exam
9)	Cell Culture
10)	Passage in Cell Culture Cloning Technology
11)	Proteomics Technology Application of Proteomics
12)	Electrophoretic Analysis of Proteins
13)	Determination of Protein Jellies by Dyeing, Membrane Transfer and Immunological Methods Purification of Proteins by Different Chromatographic Methods
14)	FINAL EXAM

Sources

Course Notes / Textbooks:	Genetic Concepts Palme Publishing House, 2008 ISBN: 9786055829261
References:	Moleküler Biyoloji Teknikleri - Damla ARISAN

Course-Program Learning Outcome Relationship

Learning Outcomes	1	2	3
Program Outcomes
1) It has knowledge of the basic structure and functioning of the human body. Describes systems and anatomical structures.
2) Describes terms related to their domain.
3) Have basic theoretical and practical knowledge including current knowledge in the field of Medical Laboratory Techniques Program.
4) To be able to use information and communication skills, to have the ability to synthesize knowledge in the technical equipment that can apply the developing technology to the field.
5) Have knowledge of basic scientific knowledge in the field of Medical Laboratory Techniques Program.
6) Describe the problems encountered in an unexpected situation related to the analysis in accordance with the information obtained in the field of Medical Laboratory Techniques Program.
7) It uses basic computer programs and related technologies related to the field.
8) He works as a team member to resolve problems during injuries and other emergencies.
9) It develops at a critical level knowledge and skills at the basic level in the field.
10) It communicates effectively with colleagues, patients, patient relatives, physicians and other health professionals.
11) The profession acquires moral consciousness and conforms to the scientific and ethical rules in the process of collecting data concerning the profession.
12) The Medical Laboratory Techniques Program independently conducts a given task using the basic knowledge of the field.
13) Have a life-long learning consciousness.
14) Using a foreign language, he tracks resources in his area and communicates with colleagues.
15) Evaluate and apply up-to-date information professionally.
16) He has experience working with other health disciplines.

Course - Learning Outcome Relationship

No Effect	1 Lowest	2 Low	3 Average	4 High	5 Highest

	Program Outcomes	Level of Contribution
1)	It has knowledge of the basic structure and functioning of the human body. Describes systems and anatomical structures.
2)	Describes terms related to their domain.
3)	Have basic theoretical and practical knowledge including current knowledge in the field of Medical Laboratory Techniques Program.
4)	To be able to use information and communication skills, to have the ability to synthesize knowledge in the technical equipment that can apply the developing technology to the field.
5)	Have knowledge of basic scientific knowledge in the field of Medical Laboratory Techniques Program.
6)	Describe the problems encountered in an unexpected situation related to the analysis in accordance with the information obtained in the field of Medical Laboratory Techniques Program.
7)	It uses basic computer programs and related technologies related to the field.
8)	He works as a team member to resolve problems during injuries and other emergencies.
9)	It develops at a critical level knowledge and skills at the basic level in the field.
10)	It communicates effectively with colleagues, patients, patient relatives, physicians and other health professionals.
11)	The profession acquires moral consciousness and conforms to the scientific and ethical rules in the process of collecting data concerning the profession.
12)	The Medical Laboratory Techniques Program independently conducts a given task using the basic knowledge of the field.
13)	Have a life-long learning consciousness.
14)	Using a foreign language, he tracks resources in his area and communicates with colleagues.
15)	Evaluate and apply up-to-date information professionally.
16)	He has experience working with other health disciplines.

Learning Activity and Teaching Methods

Lab
Application (Modelling, Design, Model, Simulation, Experiment etc.)
Internship/Onsite Practice

Assessment & Grading Methods and Criteria

Assessment & Grading

Semester Requirements	Number of Activities	Level of Contribution
Midterms	1	% 40
Final	1	% 60
total		% 100
PERCENTAGE OF SEMESTER WORK		% 40
PERCENTAGE OF FINAL WORK		% 60
total		% 100

Workload and ECTS Credit Grading

Activities	Number of Activities	Duration (Hours)	Workload
Course Hours	16	3	48
Laboratory	5	16	80
Homework Assignments	1	5	5
Midterms	1	2	2
Final	1	15	15
Total Workload			150