Genetics and Bioengineering
Bachelor	TR-NQF-HE: Level 6	QF-EHEA: First Cycle	EQF-LLL: Level 6

General course introduction information

Course Code:

GBM302

Course Name:

Genetic Engineering

Course Semester:

Fall

Course Credits:

Theoretical	Practical	Credit	ECTS
2	4	4	8

Language of instruction:

Course Requisites:

Does the Course Require Work Experience?:

Type of course:

Compulsory

Course Level:

Bachelor

TR-NQF-HE:6. Master`s Degree

QF-EHEA:First Cycle

EQF-LLL:6. Master`s Degree

Mode of Delivery:

Face to face

Course Coordinator :

Dr.Öğr.Üyesi ÖZGE ACAR

Course Lecturer(s):

Dr.Öğr.Üyesi METİN YAZAR
Dr.Öğr.Üyesi ELİF KUBAT ÖKTEM

Course Assistants:

Course Objective and Content

Course Objectives:

The purpose of this course is to provide the detailed theoretical knowledge and practical experience necessary to underline the genetic engineering methods.

At the end of this course the student will be able to:
• underline how genetic engineering can be used to edit the genome to set up experimental models
• recognize the methods of genetic engineering
• perform DNA cloning
• differentiate the vectors for protein expression
• design a recombinant DNA via using bioinformatics tools
• express a recombinant protein
• explain how genetic editing is done in cell lines
• discuss the applications of genetic engineering in different fields of industry

Course Content:

• Introduction to genetic engineering methods
• Lab Safety Rules and Biosafety Levels
• Provide an overview of DNA cloning
• Explain plasmid vectors
• Explain recombinant DNA techniques
• Explain gene libraries and screening
• Underline analysis cloned DNA
• Explain applications of cloning
• Explain reverse transcription
• Explain protein purification and analysis methods
• Explain how to make genomic modification in mammalian cell lines
• Explain transgenic animals and plants
• Explain applications of genetic engineering in industry
• Explain biosimilar production as an application of genetic engineering

Learning Outcomes

The students who have succeeded in this course;

Learning Outcomes

1 - Knowledge
Theoretical - Conceptual
2 - Skills
Cognitive - Practical
3 - Competences
Communication and Social Competence
Learning Competence
Field Specific Competence
1) At the end of this course the student will be able to know how genetic engineering can be used to edit the genome to set up experimental models
Competence to Work Independently and Take Responsibility

Lesson Plan

Week	Subject	Related Preparation
1)	• Introduction to genetic engineering methods • Lab Safety Rules and Biosafety Levels	-
2)	• What is DNA cloning? • Bacterial expression plasmids • Mammalian expression plasmids • Cosmids, YACs and BACs • Diagnostic digestion of plasmids with restriction enzymes	-
3)	• What is recombinant DNA? • Applications of recombinant DNA • PCR amplification of a region of interest	-
4)	• Genomic libraries • cDNA libraries • Screening procedures • Restriction digestion of PCR products and vector for cloning	-
5)	• Single cell cloning • Characterization of clones • Nucleic acid sequencing • PCR • Organization of cloned genes • Mutagenesis of cloned genes • Ligation of the vector and insert and transformation	-
6)	• Applications of cloning • Recombinant proteins • Genetically modified organisms • DNA fingerprinting • Medical diagnosis and therapy • Colony screening for ligation (DNA isolation and restriction digestion)	-
7)	•Midterm I	-
8)	• What is reverse transcription? • Why do we need reverse transcription? • Cell culture and preparation of cell lines for transfection	-
9)	• Protein expression • Protein purification methods • Protein analysis methods • Transfection of the cloned vector to a mammalian cell line	-
10)	• Genomic modification in cell lines by TALENs • Genomic modification in cell lines by CRISPR-Cas9 system • Design of TALEN pairs and CRISPR-Cas9 oligos via bioinformatic tools
10)	• Genomic modification in cell lines by TALENs • Genomic modification in cell lines by CRISPR-Cas9 system • Design of TALEN pairs and CRISPR-Cas9 oligos via bioinformatic tools	-
11)	• How to make a transgenic organism? • Single cell clone dilution of transfected cells	-
12)	• Midterm II	-
13)	• Genetic engineering in medicine industry • Genetic engineering in food industry • Genetic engineering in GMO • Control of protein expression via visualization of the GFP protein under fluorescent microscope	-
14)	• What is biosimilar? • Genetic engineering in biosimilar production	-
15)	• Final Exam	-

Sources

Course Notes / Textbooks:	• BIOS Instant Notes in Molecular Biology, 4th edition – Garland Science. Alexander McLennon, Andy Bates, Phil Turner and Mike White
References:	• BIOS Instant Notes in Molecular Biology, 4th edition – Garland Science. Alexander McLennon, Andy Bates, Phil Turner and Mike White

Course-Program Learning Outcome Relationship

Learning Outcomes	1
Program Outcomes
1) Sufficient knowledge in mathematics, science and engineering related to their branches; and the ability to apply theoretical and practical knowledge in these areas to model and solve engineering problems.
2) The ability to identify, formulate, and solve complex engineering problems; selecting and applying appropriate analysis and modeling methods for this purpose.
3) The ability to design a complex system, process, device or product under realistic constraints and conditions to meet specific requirements; the ability to apply modern design methods for this purpose. (Realistic constraints and conditions include such issues as economy, environmental issues, sustainability, manufacturability, ethics, health, safety, social and political issues, according to the nature of design.)
4) Ability to develop, select and use modern techniques and tools necessary for engineering applications; ability to use information technologies effectively.
5) Ability to design experiments, conduct experiments, collect data, analyze and interpret results to examine engineering problems or discipline-specific research topics.
6) The ability to work effectively in disciplinary and multidisciplinary teams; individual work skill.
7) Effective communication skills in Turkish oral and written communication; at least one foreign language knowledge; ability to write effective reports and understand written reports, to prepare design and production reports, to make effective presentations, to give and receive clear and understandable instructions.
8) Awareness of the need for lifelong learning; access to knowledge, ability to follow developments in science and technology, and constant self-renewal.
9) Conform to ethical principles, and standards of professional and ethical responsibility; be informed about the standards used in engineering applications.
10) Awareness of applications in business, such as project management, risk management and change management; awareness of entrepreneurship, and innovation; information about sustainable development.
11) Information about the universal and social health, environmental and safety effects of engineering applications and the ways in which contemporary problems are reflected in the engineering field; awareness of the legal consequences of engineering solutions.

Course - Learning Outcome Relationship

No Effect	1 Lowest	2 Low	3 Average	4 High	5 Highest

	Program Outcomes	Level of Contribution
1)	Sufficient knowledge in mathematics, science and engineering related to their branches; and the ability to apply theoretical and practical knowledge in these areas to model and solve engineering problems.	2
2)	The ability to identify, formulate, and solve complex engineering problems; selecting and applying appropriate analysis and modeling methods for this purpose.	1
3)	The ability to design a complex system, process, device or product under realistic constraints and conditions to meet specific requirements; the ability to apply modern design methods for this purpose. (Realistic constraints and conditions include such issues as economy, environmental issues, sustainability, manufacturability, ethics, health, safety, social and political issues, according to the nature of design.)	1
4)	Ability to develop, select and use modern techniques and tools necessary for engineering applications; ability to use information technologies effectively.	1
5)	Ability to design experiments, conduct experiments, collect data, analyze and interpret results to examine engineering problems or discipline-specific research topics.	1
6)	The ability to work effectively in disciplinary and multidisciplinary teams; individual work skill.	1
7)	Effective communication skills in Turkish oral and written communication; at least one foreign language knowledge; ability to write effective reports and understand written reports, to prepare design and production reports, to make effective presentations, to give and receive clear and understandable instructions.	1
8)	Awareness of the need for lifelong learning; access to knowledge, ability to follow developments in science and technology, and constant self-renewal.	1
9)	Conform to ethical principles, and standards of professional and ethical responsibility; be informed about the standards used in engineering applications.	1
10)	Awareness of applications in business, such as project management, risk management and change management; awareness of entrepreneurship, and innovation; information about sustainable development.
11)	Information about the universal and social health, environmental and safety effects of engineering applications and the ways in which contemporary problems are reflected in the engineering field; awareness of the legal consequences of engineering solutions.

Learning Activity and Teaching Methods

Expression
Brainstorming/ Six tihnking hats
Lesson
Lab
Application (Modelling, Design, Model, Simulation, Experiment etc.)

Assessment & Grading Methods and Criteria

Written Exam (Open-ended questions, multiple choice, true-false, matching, fill in the blanks, sequencing)
Homework
Application

Assessment & Grading

Semester Requirements	Number of Activities	Level of Contribution
Laboratory	12	% 10
Midterms	2	% 50
Final	1	% 40
total		% 100
PERCENTAGE OF SEMESTER WORK		% 60
PERCENTAGE OF FINAL WORK		% 40
total		% 100

Workload and ECTS Credit Grading

Activities	Number of Activities	Duration (Hours)	Workload
Course Hours	15	2	30
Laboratory	15	4	60
Midterms	2	3	6
Final	1	2	2
Total Workload			98