Techonology of Pathology Laboratory
Associate	TR-NQF-HE: Level 5	QF-EHEA: Short Cycle	EQF-LLL: Level 5

General course introduction information

Course Code:

PTL213

Course Name:

Immunohistochemistry

Course Semester:

Spring

Course Credits:

Theoretical	Practical	Credit	ECTS
2	0	2	4

Language of instruction:

Course Requisites:

Does the Course Require Work Experience?:

Type of course:

Compulsory

Course Level:

Associate

TR-NQF-HE:5. Master`s Degree

QF-EHEA:Short Cycle

EQF-LLL:5. Master`s Degree

Mode of Delivery:

Course Coordinator :

Dr.Öğr.Üyesi DİDAR AYGÜN GÜLHAN

Course Lecturer(s):

Dr.Öğr.Üyesi DİDAR AYGÜN GÜLHAN

Course Assistants:

Course Objective and Content

Course Objectives:	The immune system plays a vital role in protecting our body against infections and cancer. As a host defense system, it must be able to distinguish between external pathogens and its own tissues. This course examines the basic principles of immunology and the tissues and cells involved in the immune system.
Course Content:	Basic concepts of immunology. Antigen-antibody definition and properties. the place and importance of immunohistochemistry in the pathology laboratory. Immuno staining procedure. Manual and device immunostaining methods. Possible problems and solutions.

Learning Outcomes

The students who have succeeded in this course;

Learning Outcomes

1 - Knowledge
Theoretical - Conceptual
1) To have basic theoretical and applied knowledge supported by textbooks containing up-to-date information in the field, application tools and other resources based on the qualifications gained at secondary education level.
2 - Skills
Cognitive - Practical
1) - Gaining the skills to use the basic theoretical and applied knowledge acquired in the field at an advanced education level in the same field or in a field at the same level. - To be able to interpret and evaluate data, define and analyze problems, and develop solutions based on evidence by using the basic knowledge and skills acqıired in the field
3 - Competences
Communication and Social Competence
1) To be able to convey his thoughts through written and verbal communication at the level of basic knowledge and skills on the subjects related to his field. - To be able to share thoughts and solutions to problems related to his field with experts and non-experts. - To be able to follow the information in the field and communicate with colleagues by using a foreign language at least at the A2 General Level of the European Language Portfolio. - To be able to use information and communication technologies together with computer software at minimum European Computer License Basic Level required by the field.
2) To be able to convey his thoughts through written and verbal communication at the level of basic knowledge and skills on the subjects related to his field. - To be able to share thoughts and solutions to problems related to his field with experts and non-experts. - To be able to follow the information in the field and communicate with colleagues by using a foreign language at least at the A2 General Level of the European Language Portfolio. - To be able to use information and communication technologies together with computer software at minimum European Computer License Basic Level required by the field.
Learning Competence
1) To be able to critically evaluate the basic level of knowledge and skills acquired in the field, to identify and meet learning needs. - To be able to direct one's education to an advanced education level in the same field or to a profession at the same level. - Gaining awareness of lifelong learning
Field Specific Competence
1) - Having social, scientific, cultural and ethical values in the stages of collecting, applying and announcing the results related to the field. - Having sufficient awareness of the universality of social rights, social justice, quality and cultural values, environmental protection, occupational health and safety
Competence to Work Independently and Take Responsibility
1) - Being able to independently carry out a basic level study related to the field. - To be able to take responsibility as a team member to solve unforeseen complex problems encountered in applications related to the field. - To be able to carry out activities for the development of the employees under his/her responsibility within the framework of a project

Lesson Plan

Week	Subject	Related Preparation
1)	Explaining immune system pathologies • Information on immunodeficiency states, hypersensitivity diseases, autoimmune diseases • Immunopathology • Immunodeficiency diseases, autoimmune diseases, hypersensitivity diseases Research about the course
2)	• Teaching what is antigen. • Recognizing the properties of being antigens• • Antigen structure • Antigen properties
3)	Explaining immune system pathologies • Information on immunodeficiency states, hypersensitivity diseases, autoimmune diseases • Immunopathology • Immunodeficiency diseases, autoimmune diseases, hypersensitivity diseases
4)	• Teaching what is antigen. • Recognizing the properties of being antigens
5)	• What is an antibody, its structure is explained • Structures and properties of polyclonal and monoclonal antibodies.
6)	• Giving general information about immunohistochemistry. • Explain the stages of immunohistochemistry.
7)	What is antigen masking • Explaining how to unmask antigen.
8)	• Evaluates students with midterm exam.
9)	• What is a blockade, what are its sources, how to remove it • Enzymes and chromogens in IHC
10)	• Teaching the detection methods of antigen in IHC staining • Explaining direct, indirect method, soluble enzyme-antienzyme complex, Avidin-Biotin methods
11)	• Explaining what is counter staining in IHC staining and why it is done • Transparency and closure
12)	IHC staining procedure explanation.
13)	IHK laboratory practice
14)	• Explaining the types of immunohistochemical dyes. • Giving general information about immunohistochemical dyes.
15)	Evaluates students with the final exam.

Sources

Course Notes / Textbooks:	Yok
References:	Histokimyasal ve immünhistokimyasal yöntemler- H. Seda Vatansever. Celel Bayar üniversitesi Tıp Fakültesi. Histoloji- Embriyoloji ABD Hematoksilen boyama ve kapama yöntemi. Zeki Karabük. AÜTF Patoloji ABD

Course-Program Learning Outcome Relationship

Learning Outcomes	1	2	3	4	5	6	7
Program Outcomes
1) Possess theoretical and practical knowledge supported by textbooks with updated information, practice equipments and other resource on basic level based on health qualifications gained at secondary education level.
2) Gain the skills to use basic level theoretical and practical knowledge acquired within the field in the same field of a higher education level or in a field of same level
3) Interpret and evaluate data, define problems, do analysis, produce solutions based on proof with using basic level knowledge and practices gained within the field
4) Transfer the ideas based on the basic knowledge and skills acquired within the field through written and oral communication
5) Share the ideas and solution proposals to problems about issues within the field with professionals and non-professionals
6) Monitor the developments in the field and communicate with peers by using a foreign language at least at a level of European Language Portfolio A2 General Level
7) Use informatics and communication technologies with at least a minimum level of European Computer Driving License Basic Level software knowledge
8) Evaluate the acquired knowledge and skills at basic level within the field with a critical approach, determine and respond to learning needs
9) Direct the education received to a higher education level in the same field or to an occupation in the same level
10) Gain awareness of lifelong learning
11) Possess social, scientific, cultural and ethic values on the stages of gathering, implementation and release of the results of data related to the field
12) Possess sufficient consciousness about the issues of universality of social rights, social justice, quality, cultural values and also, environmental protection, worker's health and security
13) Conduct studies at basic level within the field independently
14) Take responsibility as a team member in order to solve unexpected complex problems faced in the implementations within the field
15) Conduct activities towards the development of subordinates within a project

Course - Learning Outcome Relationship

No Effect	1 Lowest	2 Low	3 Average	4 High	5 Highest

	Program Outcomes	Level of Contribution
1)	Possess theoretical and practical knowledge supported by textbooks with updated information, practice equipments and other resource on basic level based on health qualifications gained at secondary education level.
2)	Gain the skills to use basic level theoretical and practical knowledge acquired within the field in the same field of a higher education level or in a field of same level
3)	Interpret and evaluate data, define problems, do analysis, produce solutions based on proof with using basic level knowledge and practices gained within the field
4)	Transfer the ideas based on the basic knowledge and skills acquired within the field through written and oral communication
5)	Share the ideas and solution proposals to problems about issues within the field with professionals and non-professionals
6)	Monitor the developments in the field and communicate with peers by using a foreign language at least at a level of European Language Portfolio A2 General Level
7)	Use informatics and communication technologies with at least a minimum level of European Computer Driving License Basic Level software knowledge
8)	Evaluate the acquired knowledge and skills at basic level within the field with a critical approach, determine and respond to learning needs
9)	Direct the education received to a higher education level in the same field or to an occupation in the same level
10)	Gain awareness of lifelong learning
11)	Possess social, scientific, cultural and ethic values on the stages of gathering, implementation and release of the results of data related to the field
12)	Possess sufficient consciousness about the issues of universality of social rights, social justice, quality, cultural values and also, environmental protection, worker's health and security
13)	Conduct studies at basic level within the field independently
14)	Take responsibility as a team member in order to solve unexpected complex problems faced in the implementations within the field
15)	Conduct activities towards the development of subordinates within a project

Learning Activity and Teaching Methods

Expression
Individual study and homework
Lesson
Problem Solving
Q&A / Discussion

Assessment & Grading Methods and Criteria

Written Exam (Open-ended questions, multiple choice, true-false, matching, fill in the blanks, sequencing)
Oral Examination
Homework
Application
Observation
Individual Project

Assessment & Grading

Semester Requirements	Number of Activities	Level of Contribution
Midterms	1	% 40
Final	1	% 60
total		% 100
PERCENTAGE OF SEMESTER WORK		% 40
PERCENTAGE OF FINAL WORK		% 60
total		% 100

Workload and ECTS Credit Grading

Activities	Number of Activities	Duration (Hours)	Workload
Midterms	1	40	40
Final	1	60	60
Total Workload			100